Olink Reveal plasma proteomics services: >1,000 proteins from 4 µL
Explicyte delivers Olink Reveal NGS-based proteomics for plasma and serum biomarker studies, measuring >1,000 proteins from 4 µL with in-house data analysis and biological interpretation.
Proteomics data published in
Olink proteomics CRO services
Why teams run Olink with us
>1,000 proteins from 4 µL
Reveal profiles >1,000 proteins from 4 µL of plasma or serum, with broad pathway coverage and deep representation of inflammation and immune-response biology.
Olink-trained PEA workflow
Our team runs the PEA workflow from sample preparation and assay execution through library QC and NGS readout, with study design adapted to your cohort.
In-house proteomics analysis
Our data science team handles QC, differential protein analysis, pathway interpretation and integration with clinical or multiomic data alongside the study director.
From your objectives to an interpreted dataset
How we run Olink Reveal studies
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1Objectives, samples, and study design
A PhD study director scopes the biological question, sample matrix, cohort structure, timepoints, timeline and budget, then defines the Reveal study design around the comparisons you need to make.
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2Sample logistics and QC
We define collection, volume, storage and shipping requirements upfront. Samples are registered, checked for pre-analytical issues and organized into a plate layout designed to limit avoidable technical variation.
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3PEA library preparation and NGS readout
Reveal uses dual-antibody Proximity Extension Assay chemistry to generate protein-specific DNA barcodes, followed by library amplification, QC and NGS readout. Controls and sample allocation are planned across the batch before processing.
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4Data analysis and biological interpretation
Olink sequencing data are processed to normalized NPX values. Optional in-house analysis includes differential protein abundance, pathway and signature analysis, cohort comparisons, clinical associations and publication-ready visualization.
- Panel
- Olink Reveal — >1,000 curated human proteins with broad biological coverage and deep representation of inflammation and immune-response pathways.
- Technology
- Proximity Extension Assay (PEA) with NGS readout. Dual-antibody recognition generates protein-specific DNA barcodes for multiplex protein measurement.
- Sample types
- Human plasma and serum are the primary matrices. Other sample types can be considered case by case after feasibility review.
- Sample volume
- 4 µL assay input per sample. Additional material may be requested for handling, QC or repeat analysis.
- Readout
- Normalized Protein eXpression (NPX) values for relative protein abundance, with standardized Olink QC.
Olink Reveal data and analysis deliverables
- Olink QC outputs and normalized NPX data
- Per-sample and per-protein QC summary
- Differential protein abundance across groups or timepoints
- Pathway, signature and functional enrichment analyses
- Clinical or multiomic integration where applicable
- Publication-ready figures and biological interpretation report
Featured publication
Plasma proteomics study in Annals of Oncology
Paul Marteau, PharmD (study director), Imane Nafia, PhD (CSO), Loïc Cerf, MSc (COO), Alban Bessede, PhD (founder, CEO), Jean-Philippe Guégan, PhD (CTO)
Contact our team
Discuss your Olink Reveal project
Have a plasma or serum proteomics project in mind? Tell us the sample matrix, cohort size and biological question — we'll advise on study design, analysis and integration with complementary readouts.
Answers about Olink proteomics services
Frequently asked questions
What is Olink Reveal, and when should I use it?
Olink Reveal is an NGS-based proteomics assay measuring >1,000 human proteins from 4 µL of plasma or serum. It is suited to biomarker discovery, pharmacodynamic studies, mechanism-of-action research and cohort profiling when broad protein coverage is needed from limited sample volume.
Which Olink proteomics workflow does Explicyte offer?
Explicyte’s current Olink proteomics service is Olink Reveal, providing broad profiling of >1,000 proteins from plasma or serum with NGS readout and optional in-house data analysis.
What sample types and volumes do you accept?
Approximately 30–50 µL of plasma or serum per sample is requested for study execution, depending on QC and potential repeat-analysis requirements. The Olink Reveal assay itself uses 4 µL of sample input.
What proteins are covered by Olink Reveal?
Reveal measures >1,000 curated human proteins with broad biological pathway coverage and particularly deep representation of inflammation and immune-response pathways. We can check the current panel content against your markers of interest during study scoping.
What data do I receive?
Core outputs include Olink QC results and normalized NPX values. Optional analysis can include differential protein abundance, pathway and signature analysis, cohort or longitudinal comparisons, clinical associations and publication-ready figures.
Can Olink Reveal be integrated with single-cell or flow-cytometry data?
Yes. Plasma proteomics can be integrated with matched PBMC profiling by Chromium X or multiplex flow cytometry to connect circulating protein changes with immune-cell composition and cell states.
Can Explicyte analyze Olink data generated elsewhere?
Yes. Our data science team can analyze normalized Olink datasets generated externally, including QC review, differential analysis, pathway interpretation and integration with matched clinical or multiomic data.
Can Explicyte help with biospecimen sourcing?
For selected studies, we can explore access to plasma, serum or matched tissue through our clinical and biobank network. Feasibility depends on indication, cohort size, inclusion criteria and required metadata.
