How fucoidan activates immune cells and independently stalls prostate cancer growth alongside Nivolumab
Evaluation of ADCC activity using primary NK cells and SKOV3 ovarian tumor cells
Antibody-dependent cell-mediated cytotoxicity, or ADCC, is a key immune mechanism by which antibody-coated target cells are recognized and eliminated by immune effector cells. Several cells of the innate immune system, including natural killer cells and granulocytes, express CD16, a low-affinity Fc receptor for immunoglobulins. Upon binding to the Fc region of antibodies attached to target […]
Functional evaluation of neutrophil migration toward tumor-associated chemoattractants
Neutrophils are increasingly recognized as important contributors to the tumor microenvironment. Beyond their antimicrobial functions, tumor-associated neutrophils can infiltrate several tumor types and have been associated with poor clinical outcome. Their recruitment is driven by key chemokines produced by tumor cells or by the surrounding microenvironment, including CXCL8/IL-8 and CXCL1. By promoting the recruitment of […]
Functional evidence for an immunosuppressive role of kynurenine in cancer patients
Background Long neglected, tumor immune escape is now considered a key mechanism promoting tumor growth and immune evasion. Beyond the prototypical PD1/PDL1 axis, several additional pathways have been identified as important contributors to the immune editing process. Indoleamine 2,3-dioxygenase 1, or IDO1, is a first rate-limiting enzyme of the kynurenine pathway. This pathway has been […]
Immune Cell-Mediated Killing of H1299 Lung Cancer Cells Following Anti-CD3 Activation
The initiation of an anti-tumor immune response and the subsequent elimination of cancer cells are central mechanisms of cancer immunosurveillance. In immuno-oncology drug development, one key objective is to enhance the ability of immune effector cells to recognize, engage, and eliminate tumor cells. In this context, in vitro immune cell-mediated killing assays provide a highly […]